LOCUS p11 5704 bp DNA circular 28-APR-2005 DEFINITION p11: His tag-TEV expression vector. SOURCE ORGANISM COMMENT COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT ORIGDB|GenBank COMMENT VNTDATE|332796117| COMMENT VNTDBDATE|372966778| COMMENT LSOWNER| COMMENT VNTNAME|p11| COMMENT VNTAUTHORNAME|Opher| COMMENT VNTREPLTYPE|Plasmid FEATURES Location/Qualifiers primer_bind 1..17 /vntifkey="28" /label=T7-forward protein_bind 100..118 /vntifkey="31" /label=His\tag CDS complement(4239..5318) /vntifkey="4" /label=lac\I rep_origin complement(2302..2302) /vntifkey="33" /label=ColE1\pBR322\origin CDS 684..1541 /vntifkey="4" /label=bla\(Ap)\sequence CDS complement(20..44) /vntifkey="4" /label=lac\operator primer_bind 449..473 /vntifkey="28" /label=pET-rev protein_bind 148..148 /vntifkey="31" /label=TEV\cleavage BASE COUNT 1345 a 1501 c 1549 g 1309 t ORIGIN 1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt 61 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc 121 agcggcagag aaaacttgta tttccagggc catatggcta gcttgactgg tggacaggga 181 tcctaataac taagtaaact agtgctgagc aataactagc ataacccctt ggggcctcta 241 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat cccgcaagag 301 gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag 361 gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa 421 ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt 481 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 541 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 601 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 661 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 721 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 781 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 841 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 901 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca 961 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 1021 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 1081 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 1141 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 1201 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa 1261 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 1321 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 1381 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 1441 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 1501 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 1561 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 1621 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 1681 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 1741 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 1801 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 1861 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 1921 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 1981 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 2041 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 2101 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 2161 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 2221 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 2281 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 2341 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 2401 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 2461 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt 2521 gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata 2581 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac 2641 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga 2701 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa 2761 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct 2821 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg 2881 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa 2941 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata 3001 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg 3061 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat 3121 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata 3181 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc 3241 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg 3301 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc 3361 aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc 3421 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc 3481 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta 3541 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca 3601 acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg 3661 tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc 3721 tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg 3781 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg 3841 ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg 3901 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga 3961 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg 4021 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta 4081 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc 4141 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct 4201 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 4261 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 4321 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc 4381 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga 4441 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg 4501 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt 4561 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc 4621 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct 4681 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc 4741 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga 4801 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc 4861 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg 4921 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga 4981 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg 5041 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc 5101 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt 5161 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt 5221 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg 5281 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc 5341 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg 5401 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag 5461 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc 5521 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg 5581 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt 5641 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg 5701 aaat //